George L. Holt (fl. c. 1867 - c. 1910) was a Wyoming druggist, businessman, and publisher based in Cheyenne, Wyoming during the second half of the 19th century. He was one of the earliest pioneers to Wyoming, arriving in Cheyenne in September of 1867. Holt ran a drugstore on Main Street, Cheyenne, upon which the entire region relied in times of need. In the 1880s he and his wife relocated to Buffalo, Wyoming, where his drugstore continued to operate until roughly 1920. Along with Peter Murray, he was also a shareholder in the 'Cross H Ranch', located just south of Buffalo. Holt was a board member of the First National Bank of Buffalo, the first such bank charted in Wyoming Territory. Holt one important map of Wyoming from 1883 to 1888, but no other known maps. He was survived by his son, Wilbur R. Holt.

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